VEGFR2抗体说明书

Rabbit Anti- VEGFR2Catalog Number : RS-0565R Quantity size :   0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background:   VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi’s sarcoma lesions. Specificity : – anti- VEGFR2 is a rabbit monoclonal antibody unconjugated – specific for VEGFR2 of rat   – epitope mapping near the amino term inus of VEGFR2  of rat origin –  use for elisa, immunoprecipitation and immunohistochemistry– Protein G affinity chromatogr aphy purification, purity :>95%– Isotype: IgG – mol wt: 151kDa  Rabbit Anti- VEGFR2Application : – Western blotting 1:100-500  – Immunohistochemistry 1:100-500  –  ELISA 1:500-1000  –  Optimal working dilutions must be determined by the end user.  Storage:   Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃. Important Note:This product as supplied is intended for research use only, not for use in therapeutic or diagnostic applications.

Rabbit Anti- VEGFR2